Periodic Acid Silver Methenamin Stain Kit

Description The dialdehydes formed by treatment with periodic acid from certain carbohydrates, will selectively reduce an alkaline hexamine-silver salt mixture. This kit is especially suitable for renal glomerular basement membranes. It is very helpful to determine the thickness of glomerular basement membranes and mesangial matrix. Kit Components Sku# Reagent Name Volume (ml) VB-3033-1 Periodic acid 1% 100 ml VB-3033-2 Silver nitrate 5% 35 ml VB-3033-3 Methenamine 3% 35 ml VB-3033-4 Sodium tetraborate 2% 35 ml VB-3033-5 Gold chloride  0.25% 100 ml VB-3033-6 Sodium thiosulfate 5% 100ml VB-3033-7 Mayer’s hematoxylin solution 100 ml VB-3033-8 Eosin solution 100 ml Storage  Store Silver Nitrate 5% and Gold chloride 0.25% at 2-8 °C. The other components can be stored at room temperature. Protocol Deparaffinize in xylene I for 6 minutes and II for 6 minutes. Rehydrate: Ethanol 100% (2 minutes)×2 Ethanol 95% (2 minutes)×2 Ethanol 70% (2 minutes)×1 Rinse in DI water (2 minutes)×1 Incubate the sections with Periodic acid 1% for 30 minutes. Wash in distilled water. Fresh make silver methenamine solution: Mix Silver nitrate 5% , Methenamine 3% and Sodium tetraborate 2% at a ratio of 1:1:1. Incubate the section with silver methenamine solution in oven for 30 minutes at 60°C. Check the slide microscopically after 20 minutes then every 5 minutes until the basement membrane is well demonstrated. If necessary, incubate again. The sections should turn tobacco. Wash in hot distilled water (60°C) for 4 min. Wash in room temperature distilled water for 1 min. Incubate the section with Gold chloride 0.25% for 1 minute. Wash in distilled water. Incubate the section with Sodium thiosulfate 5% for 1 minute. Wash in distilled water. Stain the sections with hematoxylin for 2 min. Running tap water for 2 min. Stain the sections with Eosin for 1min. Dehydrate with 2 changes of 100% Ethanol (2 minute per change). Clear with 2 changes of xylene (5 minute per change). Mount cover slip onto glass slide with Permount or some other suitable organic mounting medium. Control Tissue: Kidney section  Expected Results: Basement membranes …………………………….Black Cell Nuclear…………………………………………Blue Background ………………………………………..Pink/Red References: Ohga S, et al (2007) . Thiazolidinedione ameliorates renal injury in experimental diabetic rats through anti-inflammatory effects mediated by inhibition of NF-kappaB activation. American journal of physiology. Renal physiology 292(4):F1141-50. Francis A, at al.(2016). Polypoid Change of the Glomerular Basement Membrane in a Child with Steroid Resistant Nephrotic Syndrome and ARHGAP24 Mutation: A Case Report. The Open Urology & Nephrology Journal 9(1):88-93 Note: This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. Precautions: Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves. More Images Human Kidney, Periodic Acid Silver Methenamin Stain Human Kidney, Periodic Acid Silver Methenamin Stain Human Kidney, Periodic Acid Silver Methenamin Stain User Manual and Material Safety Data Sheet (MSDS)  (PDF) VB-3033-User Manual VB-3033 MSDS